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What is the stationary phase in TLC chromatography?

The silica gel (or the alumina) is the stationary phase. The stationary phase for thin layer chromatography also often contains a substance which fluoresces in UV light – for reasons you will see later. The mobile phase is a suitable liquid solvent or mixture of solvents.

Which of the following solvents would be the best to separate a mixture containing 2 phenylethanol and acetophenone by TLC?

methyl chloride

Why should the TLC plate be handled with gloves?

Aluminum and plastic plates are also flexible, which may result in flaking of the stationary phase. Never under any circumstances touch the face of a TLC plate with your fingers as contamination from skin oils or residues on gloves can obscure results. Instead, always handle them by the edges, or with forceps.

What happens if you touch the front of a TLC plate with your finger s )?

Nothing Will Happen The Chemicals On Your Finger Will Alter The Acidic Alumina And Turn It Into Silica Oils And Grease From Your Finger Will Transfer To The TLC And Will Interfere With Functioning Of TLC The TLC Powder Will All Fall Off Leaving A Blank TLC Plate How Can You Avoid …

Why is a pencil needed for the TLC experiment?

Pencil is always used to mark chromatography paper or TLC plates because ink may run and interfere with the chromatogram. As soon as the paper/plate is taken out, mark the solvent front with a pencil before the solvent evaporates and the front becomes impossible to see.

Why is filter paper used in a TLC developing jar?

It’s used to test for the presence of various materials, to monitor the rate and progress of a reaction or to determine the purity of a product. Filter paper impregnated with solvent is usually used to saturate the development chamber’s air with solvent vapor so the stationary phase doesn’t dry during the process.

What is the purpose of this Wick?

The purpose of a wick is to deliver fuel (wax) to the flame. Acting like a fuel pump, the wick draws the liquefied wax up into the flame to burn.

How can TLC be used to identify components in a mixture?

TLC can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound. Development consists of placing the bottom of the TLC plate into a shallow pool of a development solvent, which then travels up the plate by capillary action.

How does the liquid rise through the filter paper?

How does the liquid rise through the filter paper ? Answer. By means of capillary action.

What is the basic principle of chromatographic process?

Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase (stable phase) is separating from each other while moving with the aid of a mobile phase.

What are the moving and stationary phases in paper chromatography?

In paper and thin-layer chromatography the mobile phase is the solvent. The stationary phase in paper chromatography is the strip or piece of paper that is placed in the solvent. Both these kinds of chromatography use capillary action to move the solvent through the stationary phase.