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Should a standard curve go through zero?

As Adam stated, the origin does not have to go through zero due to the absorbance of the reagent itself, but also due to possible small contaminants in your vials, water, etc. Nonetheless, a zero origin for your standard curve will not effect results, provided your samples are suitably diluted in the same media.

Why is the line forced through zero in the standard curve?

The decision to force zero or not on the calibration curve is based upon how close the calculated y-intercept is to zero. If y is less than one standard deviation (SD) away from zero, it can be assumed that this is normal variation and x = 0, y = 0 can be used in the standard curve.

What is the Y-intercept in Beer’s law?

Note that Beer’s Law is the equation for a straight line with a y-intercept of zero.

Why do you set the Y-intercept to zero?

The y-intercept is what y equals when x equals 0. If the y-intercept is 0, then that means the line passes through the point (0,0) which is also the origin. So, if the y-intercept is 0, then that means the line passes through the origin.

What happens if Y intercept is 0?

If a line has no y-intercept, that means it never intersects the y-axis, so it must be parallel to the y-axis. This slope of this line is undefined. If the line has no x-intercept, then it never intersects the x-axis, so it must be parallel to the x-axis. This means it is a horizontal line, such as .

What happens when y equals 0?

To review, there are two methods you can use to graph y=0: the slope intercept form and plugging in values. But you can also remember a shortcut, which is that a slope of zero will always be represented as a horizontal line and therefore, when y=0, the graph will essentially show a line through the x-axis.

What is it called when the Y intercept is 0?

an x-intercept is a point in the equation where the y-value is zero, and.

What if the slope is zero?

Since we did not have a change in the x values, the denominator of our slope became 0. This means that we have an undefined slope. If you were to graph the line, it would be a vertical line, as shown above. The slope of the line is undefined.

What does the Y-intercept represent on a graph?

The y -intercept of a graph is the point where the graph crosses the y -axis. For example, we say that the y -intercept of the line shown in the graph below is 3.5 . When the equation of a line is written in slope-intercept form ( y=mx+b ), the y -intercept b can be read immediately from the equation.

What does Slope tell us in a regression line?

The slope indicates the steepness of a line and the intercept indicates the location where it intersects an axis. The slope and the intercept define the linear relationship between two variables, and can be used to estimate an average rate of change.

How do you find the slope intercept?

In general, the slope intercept form assumes the formula: y = mx + b.

  1. M is the slope (lesson on slope) Mnemonic : ‘m’ means ‘move’
  2. B is the y-intercept (lesson on the y-intercept) Mnemonic: ‘b’ means where the line begins.