Specific Aseptic Techniques

Spectrophotometry is an indirect method for calculating cell concentrations by measuring the changes in turbidity. Bacteria can also be counted by using the plating method, which is based on the number of colonies formed in Petri dishes containing specific growth media.

1. Always wipe your hands and work area with 70% ethanol.
2. It is recommended to wear gloves.
3. Wipe the outside of the containers, flasks, plates, and dishes with 70% ethanol before placing them in the cell culture hood.
4. Avoid pouring media and reagents directly from bottles or flasks.

The size of a population of microorganisms in liquid culture may be measured by counting cells directly or by first diluting the original sample and then counting cell numbers (see below), or by taking some indirect method such as the turbidity (cloudiness) of the culture.

Aseptic technique is a collection of medical practices and procedures that helps protect patients from dangerous germs. Bacteria, viruses, and microorganisms are everywhere, so using aseptic technique can help keep important equipment from being contaminated.

If something is aseptic it is sterile, sanitized, or otherwise clean of infectious organisms. The adjective aseptic, “a-” plus the root “septic,” describes anything that is not septic or is without sepsis.

In this page you can discover 29 synonyms, antonyms, idiomatic expressions, and related words for aseptic, like: barren, clean, lifeless, purifying, restrained, shrinking, sterile, arid, colorless, drab and dry.

Standard Precautions are used for all patient care. They’re based on a risk assessment and make use of common sense practices and personal protective equipment use that protect healthcare providers from infection and prevent the spread of infection from patient to patient.

Standard precautions are meant to reduce the risk of transmission of bloodborne and other pathogens from both recognized and unrecognized sources. They are the basic level of infection control precautions which are to be used, as a minimum, in the care of all patients.

The most important way to reduce the spread of infections is hand washing – frequently wash hands with soap and water, if unavailable use alcohol-based hand sanitizer (containing at least 60% alcohol). Also important is to get a vaccine for those infections and viruses that have one, when available.

Keep the germs away:

1. Wash your hands before eating, or touching your eyes, nose or mouth.
2. Wash your hands after touching anyone who is sneezing, coughing or blowing their nose.
3. Don’t share things like towels, lipstick, toys, or anything else that might be contaminated with respiratory germs.

In 1987, the practice of universal precautions was adjusted by a set of rules known as body substance isolation. In 1996, both practices were replaced by the latest approach known as standard precautions. Use of personal protective equipment is now recommended in all health care settings.

5 Steps of Universal Precautions

• Education.
• Hand washing.
• Use of protective barriers (Personal Protective Equipment (PPE))
• Cleaning of contaminated surfaces.
• Safe handling/disposal of contaminated material.

Pathogens can be transmitted a few ways depending on the type. They can be spread through skin contact, bodily fluids, airborne particles, contact with feces, and touching a surface touched by an infected person.

The Main Routes Infection can Enter the Body are:

• Body fluids – A body fluid e.g. blood, urine, pus, saliva from one person enters the body of another e.g. through cuts or other means such as sexual intercourse (e.g. syphilis, HIV etc.).
• Through the air – e.g. tuberculosis.