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How do you dilute 2X?

2X means it’s twice concentrated. If you want to use it, you have to dilute it twice. mix one volume of the 2X solution with one volume of distilled water. 20X means it’s 20 times concentrated.

How do you convert 6X to 1X?

Dilute one part 6X Dye solution into five parts of sample solution to give a final concentration of 1X Dye solution. The sample is then ready to load to a gel. For Example: 10µl sample and 2µl 6X Dye Solution. Mix equal volumes of 2X Dye Solution and RNA sample solution to give a final concentration of 1X Dye solution.

What is a 5X buffer?

5x PCR Buffer RED offers the same specification as Ammonium Buffer. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.

How do I make my 1X buffer 4x?

Add Reducing Agent Laemmli sample buffer: Add 50 µl of 2-mercaptoethanol per 950 µl (final concentration of 355 mM). 4x Laemmli sample buffer: Add 100 µl of 2-mercaptoethanol per 900 µl (final concentration of 355 mM). Alternatively, add dithiothreitol (DTT or Cleland’s reagent) to a final concentration of 50 mM.

What is the role of bromophenol blue in the sample buffer?

Bromophenol blue (BPB) is added to the sample buffer as a tracking dye that moves in the same direction of separating proteins and demarcates their leading edge.

What is a SDS buffer?

This buffer is used for the preparation and loading of protein samples onto a gel for SDS-PAGE analysis. SDS contained in the sample buffer is used to denature proteins and make them negatively charged. In this manner each protein will migrate in the electroporetic field in a measure proportional to its lenght.

Is SDS a detergent?

Sodium Dodecyl Sulfate, Molecular Biology Grade (SDS), is a detergent that is known to denature proteins. It is used in denaturing polyacrylamide gel electrophoresis for the determination of protein molecular weight.

Why SDS is used in DNA extraction?

Sodium Dodecyl Sulfate (SDS) is an anionic detergent that denatures secondary and nondisulfide-linked tertiary protein structure, shattering the native shape. SDS provides a negative charge to each protein as a function of their size. Furthermore, SDS can be used to aid in lysing cell during DNA extraction.

Why is bromophenol blue used in SDS PAGE?

It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel.

Why is bromophenol blue a good indicator?

As an acid–base indicator, its useful range lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to phenolphthalein (a popular indicator).

Does bromophenol blue glow under UV light?

Catalysts were able to degrade bromophenol blue as a harmful organic substance under UV and visible lights, although, this ability was more significant when the samples were used under visible light.

Is bromophenol blue a protein?

Bromophenol blue (BPB), a phenolphthalein anionic dye, binds to proteins under neutral to acidic conditions….Bromophenol Blue (BPB) Protein Assay.

Product Catalog # Price (USD)
Bromophenol Blue (BPB) Protein Assay Kit 6026 105.00

Is bromophenol blue positive or negative?

The dyes with negative charge are bromophenol blue, orange G and xylene cyanol. We know this because they move towards the positive electrode. The comb would be placed near the end of the gel since DNA has a negative charge and will migrate towards the positive electrode only.

Is bromophenol blue a liquid?

Bromophenol Blue, 1% (w/v), Volumetric Concentration: 1% Aqueous Composition: Water: 99%, Bromophenol Blue, Sodium Salt 1% Boiling Point: 100°C Density: 1 Melting Point: 0.0°C Color: Dark reddish-purple liquid Physical State: Liquid pH Range: 3.0 (yellow) – 4.6 (blue) Solubility Information: Miscible Shelf Life: 24…

Is bromophenol blue a weak acid?

Explanation: The idea here is that bromophenol blue acts as a weak acid in aqueous solution. As you can see, this equilibrium is influenced by the pH of the solution, i.e. by the concentration of hydronium cations present in the solution.

What is the best indicator for HCl and NaOH?

Methyl orange

Which indicator is best for strong acid and strong base?

phenolphthalein indicator

Is acetic acid a strong acid?

Strong acids are 100% ionized in solution. Weak acids are only slightly ionized. Phosphoric acid is stronger than acetic acid and so is ionized to a greater extent….Strong and Weak Acids and Acid Ionization Constant.

Acid Conjugate Base
CH 3 COOH (acetic acid) CH 3 COO − (acetate ion)

What is the pH of 1% acetic acid?

It is 0.026M. So, now we know that a 1 M acetic acid solution has a pH of 2.38.

What is the pH level of acetic acid?

2–3

What is the pH of 30% vinegar?

2.8

Is a pH of 6 acidic or basic?

Each number represents a 10-fold change in the acidity/basicness of the water. Water with a pH of five is ten times more acidic than water having a pH of six. As this diagram shows, pH ranges from 0 to 14, with 7 being neutral. pHs less than 7 are acidic while pHs greater than 7 are alkaline (basic).

Is a pH of 15 possible?

But the scale does not have fixed limits, so it is indeed possible to have a pH above 14 or below zero. For example, concentrated hydrochloric acid can have a pH of around -1, while sodium hydroxide solution can have a pH as high as 15.

What does a pH of 14 mean?

pH is a measure of how acidic/basic water is. The range goes from 0 to 14, with 7 being neutral. pHs of less than 7 indicate acidity, whereas a pH of greater than 7 indicates a base. The pH of water is a very important measurement concerning water quality.