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How can you tell if your gel electrophoresis is running properly?

How can one tell if their gel electrophoresis is running properly? It bubbles. You can see the methyl blue move from the well into the gel. The DNA runs to red.

What would happen if you left the gel accidentally in the gel electrophoresis chamber for too long?

What causes the DNA fragments to move within the gel? What would you expect to happen if you left the gel accidentally in the gel electrophoresis chamber for too long? the DNA strands would not stop they would continue to move through the gel into the buffer. In what situation do scientists need to use the PCR reaction …

What is an error in performing and evaluating gel electrophoresis?

It is important that sources of errors in this technique be minimized in order to get accurate results. One source of error is contamination of the DNA sample. If there is foreign DNA in the sample, the gel will have more bands than would be found in a gel that contains only the purified sample.

What is one reason that polyacrylamide gels have higher resolution than agarose gels for small fragments?

What is one reason that polyacrylamide gels have higher resolution that agarose gels for small fragments? Polyacrylamide gel properties can be altered by adjusting T and C values.

What is the difference between PCR and gel electrophoresis?

The results of a PCR reaction are usually visualized (made visible) using gel electrophoresis. Gel electrophoresis is a technique in which fragments of DNA are pulled through a gel matrix by an electric current, and it separates DNA fragments according to size. Right lane: result of PCR reaction, a band at 400 bp.

Why is buffer used in gel electrophoresis instead of water?

Rather than just use water, we use buffered solutions which allow the DNA to run smoothly through the gel. These solutions optimize the pH and ion concentration of the gel and will also bathe the gel as it is subjected to the electric current which actually moves the DNA through the gel.

What does the buffer solution do in gel electrophoresis?

Buffers in gel electrophoresis are used to provide ions that carry a current and to maintain the pH at a relatively constant value. Several buffers are commonly used for electrophoresis with equal success. Any buffer such as sodium borate (SB), Tris base/acetic acid/EDTA (TAE), or Tris/borate/EDTA (TBE) will be fine.

Why are some bands thicker in gel electrophoresis?

Thicker bands in gel electrophoresis mean there is more of that particular size molecule in the sample.