In this video, you will learn how to Generate a Standard Curve and determine Unknown Concentrations in Excel by a Simple Method. A standard curve is used to accurately determine the concentration of your sample from the signal generated by an assay. I will show you how to create a standard linear curve using Microsoft Excel and how to use it to calculate unknown sample values. I will use imaginary data of concentration and absorbance as an example. I have used excel 2016 to generate a standard curve and determine an unknown concentration of compound. You can also use other versions of excel such as excel 2013, excel 2019 and excel 365.

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